Fig. 3
Accumulation and decay of Bopta and Meb liver concentrations. a Normal livers (n = 9) perfused with Krebs-Henseleit bicarbonate solution (KHB) + 200 μM Bopta (left panel) and KHB (right panel). b Normal livers (n = 9) perfused with KHB + 64 μM Meb (left panel) and KHB (right panel). c Cholestatic livers lacking Mrp2 (n = 6) perfused with KHB + 200 μM Bopta (left panel) and KHB (right panel). Bopta accumulation in normal liver (a) fitted a segmental linear regression, whilst MEB accumulation (b) fitted a continuous hinge function (gentle connections between the two lines). Both regressions are described by two lines L1 and L2 intersecting at T0 or time when bile excretion started to impact the accumulation. In livers lacking Mrp2, Bopta accumulation (c) fitted a single linear regression. During the rinse period, liver concentration decay fitted a one-phase decay for Bopta and Meb (a, b, right). No Bopta decay was observed in the absence of Mrp2 (c, right)