Fig. 1
a Perfusion of imaging agents. Livers (n = 15) were successively perfused with Krebs-Henseleit bicarbonate solution (KHB) + 200 μM 153Gd-Dtpa, KHB, KHB + 200 μM 153Gd-Bopta, and KHB. Nine additional livers were perfused with KHB + 64 μM 99mTc-Dtpa, KHB, KHB + 64 μM 99mTc-Meb, and KHB. Illustration of Bopta and Meb transport across Oatp, Mrp2, and Mrp3 (b) and quantification of hepatocyte concentration (c). Extracellular concentration (CEC) is quantified during Dtpa perfusion. CHC78% for hepatocyte concentration measured by the counter. In situ concentration in bile canaliculi is similar to concentration in common bile duct (Cbile). Bile canaliculi concentration measured by the counter (CBC) is Cbile · 0.0043 (volume ratio of canaliculi to liver). CHC78% is defined by Cliver − CEC − CBC. Liver flow rate (QH), bile excretion rate (Qbile), concentration in portal vein (Cin), and concentration in hepatic veins (Cout)