Contrast-enhanced ultrasound for ovary assessment in a murine model: preliminary findings on the protective role of a gonadotropin-releasing hormone analogue from chemotherapy-induced ovarian damage

European Radiology Experimental

Table 1 Variation of ultrasound and contrast-enhanced ultrasound parameters of ovaries in decapeptyl-treated mice versus controls

	Day 0			Day 10			Day 20
	Decapeptyl	Controls	p value	Decapeptyl	Controls	p value	Decapeptyl	Controls	p value
Ultrasound (B-mode)
Ovary diameter (mm)	2.427 ± 0.70	2.039 ± 0.38	0.190	2.805 ± 0.66	2.260 ± 0.50	0.102	2.470 ± 0.77	2.183 ± 0.48	0.456
Dominant follicle diameter (mm)	0.242 ± 0.07	0.183 ± 0.06	0.248	0.659 ± 0.07	0.170 ± 0.05	< 0.001	0.430 ± 0.13	0.235 ± 0.05	0.070
Contrast-enhanced ultrasound
Peak enhancement (a.u.)	80.95 ± 6.86	41.8 ± 9.475	0.042	87.25 ± 0.21	88.60 ± 57.56	0.981	17.10 ± 18.67	145.0 ± 12.73	0.015
Wash-in-rate (a.u.)	27.83 ± 15.84	29.67 ± 14.00	0.643	58.87 ± 48.04	66.25 ± 68.94	0.561	9.09 ± 10.35	98.65 ± 28.78	0.054
Time to peak (s)	5.22 ± 2.02	4.07 ± 1.85	0.611	9.94 ± 8.10	4.04 ± 2.00	0.422	4.19 ± 0.67	3.04 ± 0.62	0.219
Rise time (s)	4.27 ± 1.66	3.65 ± 1.58	0.739	9.125 ± 7.66	3.460 ± 1.39	0.412	3.45 ± 0.74	2.545 ± 0.49	0.284
Area under the curve (a.u.)	3145.00 ± 657.61	2120.00 ± 735.39	0.279	5045.00 ± 2538.51	2450.00 ± 1088.94	0.315	362.50 ± 195.87	4975.00 ± 473.76	0.006
Mean transit time (s)	41.76 ± 25.51	59.60 ± 17.34	0.499	76.13 ± 26.51	27.34 ± 0.23	0.121	42.79 ± 33.60	55.82 ± 14.40	0.664
Perfusion index (a.u./s)	86.70 ± 37.19	35.30 ± 1.98	0.190	64.40 ± 10.89	89.35 ± 39.10	0.476	14.84 ± 16.21	91.20 ± 15.27	0.040
Wash-in perfusion index (a.u./s)	54.60 ± 3.82	29.15 ± 7.00	0.457	62.80 ± 4.24	62.25 ± 43.06	0.987	11.35 ± 12.23	97.7 ± 8.91	0.015
Wash-in area under the curve (a.u.)	230.00 ± 73.54	100.65 ± 20.29	0.139	589.50 ± 519.72	185.50 ± 62.93	0.389	34.65 ± 33.87	246.50 ± 24.75	0.019